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Praveen Patidar ¹, Sameer Singh ¹, Darshan Dubey ¹, Kamlesh Dashora ¹

Affiliations
1 Institute of Pharmacy, Vikram University, Ujjain (M.P.), IN

Abstract

Tephrosia purpurea Linn (Family-Fabeaceae) having anti-hepatotoxic activity, CNS depressant activity and it also show anti-allergic activity. The plant Tephrosia purpurea contains rutin, quercetin, rotenoids, rotenone, tephrosin and lupeol. The powdered aerial part of Tephrosia purpuria was extracted with 95% ethanol by hot continuous extraction method. The prepared extracts were subjected to HPTLC analysis. The marker compounds were estimated by using pre-coated HPTLC aluminium silica gel 60 F254 plates (MERCK) and compared with standard. The methanolic solution of standard rutin 5 μl (1 mg/ml) and Tephrosia purpuria extract 8 μl (10 mg/ml) were applied as 7mm band width using CAMAG Linomat IV applicator. The Mobile phase is ethyl acetate: formic acid: glacial acetic acid: water (100:11:11:26). The detection was carried out at 366 nm. The amount of rutin was estimated by the comparing the peak area of standard and the same was present in the extracts. The content of rutin in Tephrosia purpurea extracts was found to be 1.40% w/w. This estimation technique is very much useful for the estimation of quercetin and rutin present in the various medicinal plants.

Keywords

Rutin, HPTLC, Tephrosia purpuria

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Praveen Patidar ¹, Anis Shaikh ¹, Darshan Dubey ¹, Kamlesh Dashora ¹

Affiliations
1 Institute of Pharmacy, Vikram University, Ujjain (M.P.), IN

Abstract

Tylophora indica (Family- Asclepiadaceae) having anti-asthmatic, antiinflammatory, sedation decreased motor activity and also useful in allergic rhinitis patients. Tylophora indica contains tylophorine, -amyrin, querceitin, and other major alkaloids like tylophorinine, tylophorinidine and dehydrotylophorine. Tephrosia purpurea Linn (Family- Fabeaceae) having anti-hepatotoxic activity, CNS depressant activity and it also show antiallergic activity. The plant Tephrosia purpurea contains rutin, quercetin, rotenoids, rotenone, tephrosin and lupeol. The powdered aerial part of Tylophora indica and Tephrosia purpuria were extracted with 95% ethanol by hot continuous extraction method individually. The prepared extracts were subjected to HPTLC analysis. The marker compounds were estimated by using pre-coated HPTLC aluminium silica gel 60 F254 plates (MERCK) and compared with standard. The methanolic solution of standard quercetin 5 μl (1 mg/ ml), Tephrosia purpuria extract and Tylophora indica extract 8 μl each (10 mg/ ml) were applied as 7mm band width using C AMAG Linomat IV applicator. The Mobile phase is ethyl acetate: formic acid: glacial acetic acid: water (100:11:11:26). The detection was carried out at 366 nm. The amount of quercetin was estimated by the comparing the peak area of standard and the same was present in the extracts. The content of quercetin in extracts was found to be 4.30% w/w and 1.56% w/w for Tylophora indica extract and Tephrosia purpuria extract respectively. This estimation technique is very much useful for the estimation of quercetin present in the various medicinal plants.

Keywords

Quercetin, HPTLC, Tylophora indica and Tephrosia purpuria.

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Praveen Patidar ¹, Kamlesh Dashora ¹

Affiliations
1 Vikram University, Ujjain (M. P.), IN

Abstract

Ayurvedic formulation is essential in order to assess the quality of drugs, based on the concentration of their active principles. The main objective of the present study is to develop a simple, rapid and validated UV fingerprint method for estimation of total polyphenols as tannic acid in Manibhadra yoga and crude drugs. Tannic acid is one of the major constituent of Manibhadra yoga and it is official in Ayurvedic formulary of India and it is the most common formulation used for respiratory disorders in Ayurvedic medicinal preparation. It content of the well documented medicinal important plants Embelia ribe (Vidanga), Embelica officinalis (Amalaki), Terminalia chebula (Haritaki), Operculina turpethum (Nishotha) and guda (jeggary). The detection wavelength of tannic acid was 277.2 nm. This method is used for the estimation of tannic acid in formulations and identifies and assessed its quality by using UV method. The estimation was carried out with three laboratory batches and one marketed formulation.

Keywords

Manibhadra yoga, UV, Tannic Acid, Ayurvedic.

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Praveen Patidar ¹, Kamlesh Dashora ¹

Affiliations
1 Institute of Pharmacy, Vikram University, Ujjain (M. P), IN

Abstract

Selective and efficient analytical methods are required not only for quality assurance but also for authentication of herbal formulations. The main objective of the present study is to develop a simple, rapid and validated UV fingerprint method for estimation of total polyphenols as tannic acid in Astangavaleha and crude drugs. Astangavaleha is official in Ayurvedic formulary of India and it is the most common formulation used for respiratory disorders in Ayurvedic medicinal preparation. It Composition of the medicinal important plants Myrica esculenta, Inula racemosa, Pistacia integerrima, Trachyspermum ammi, Carum carvi, Zingiber officinale, Piper nigrum, Piper longum, madhu and Zingiber officinale (svarasa). The estimation was carried out with three laboratory batches and one marketed formulation by spectrophotometric approach at 277.2 nm.

Keywords

Astangavaleha, UV, Tannic Acid, Ayurvedic.

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